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KMID : 0624620120450100554
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BMB Reports
2012 Volume.45 No. 10 p.554 ~ p.559
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Physiological roles of N-acetylglucosaminyltransferase V (GnT-V) in mice
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Miyoshi Eiji
Terao Mika
Kamada Yoshihiro
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Abstract
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Oligosaccharide modification by N-acetylglucosaminyltransferase- V (GnT-V), a glycosyltransferase encoded by the Mgat5 gene that catalyzes the formation of β1,6GlcNAc (N-acetylglucosamine) branches on N-glycans, is thought to be associated with cancer growth and metastasis. Overexpression of GnT-V in cancer cells enhances the signaling of growth factors such as epidermal growth factor by increasing galectin-3 binding to polylactosamine structures on receptor N-glycans. In contrast, GnT-V deficient mice are born healthy and lack β1,6GlcNAc branches on N-glycans, but develop immunological disorders due to T-cell dysfunction at 12-20 months of age. We have developed Mgat5 transgenic (Tg) mice (GnT-V Tg mice) using a β-actin promoter and found characteristic phenotypes in skin, liver, and T cells in the mice. Although theGnT-V Tg mice do not develop spontaneous cancers in any organs, there are differences in the response to external stimuli between wild-type and GnT-V Tg mice. These changes are similar to those seen in cancer progression but are unexpected in some aspects. In this review, we summarize what is known about GnT-V functions in skin and liver cells as a means to understand the physiological roles of GnT-V in mice.
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KEYWORD
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EGF-R, EMT, GnT-V, NASH, Oligosaccharide
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